Multiphoton fluorescence lifetime microscope with tunable TiSapph laser (~680-1080 nm) for excitation. Two channel FLIM imaging plus a third intensity PMT. Inverted configuration with 20X, 0.8 NA air objective, 40X 1.1 NA water objective, and 100X 1.46 NA oil objective. Emission filters include 447/60 nm, 550/88 nm, and 675/67 nm. Environmental control for live cell and in vivo imaging.

Lightsheet fluorescence microscope with integrated and co-aligned infrared laser. Lightsheet includes 375, 405, 488, 561, and 640 nm excitation lasers. A 10X 0.3 water dipping objective is used for illumination and a 20X water dipping objective is used for imaging. Two cameras allow separate, two color simultaneous imaging. Emission filters include 447/60, 525/50, 513/17, 550/88, 607/70, and 692/40 nm. Infrared light (1470 nm, 1940 nm) integrated into inverted objective (20X IR objective).

Customized lightsheet microscopes for fluorescence and scattering tomography.

Custom built diffuse reflectance and fluorescence hyperspectral microscope. Fluorescence excitation at 385 nm.

Inverted widefield fluorescence microscope with an image splitter for dual picture imaging on a single camera. Currently set up for simultaneous imaging of NADH and FAD. Excitation light provided by a white light LED (X-Cite) through a 357/44 nm filter. Image splitter contains a 496 nm dichroic and 415/106 nm and 560/94 nm emission filters. Objectives include 20X, 40X 1.2 NA, and 63X. Includes perfusion system for live neuron imaging.

Fully equipped tissue culture facility adjacent to the microscopy rooms.