Reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are two co-enzymes that are used in metabolic reactions. These molecules, NADH and FAD, are fluorescent which enables label-free imaging these molecules on fluorescence microscopes. The fluorescence intensity and lifetime of NADH and FAD provide information about these molecules and their use in cells. We are currently working to develop new imaging methods for capturing metabolic dynamics within seconds, imaging mitochondria structure and movement concurrently with function, and improving specificity of metabolic interpretations from autofluorescence lifetime data.
Recent Publications:
- Autofluorescence Imaging to Evaluate Cellular Metabolism. Anna Theodossiou, Linghao Hu, Nianchao Wang, Uyen Nguyen, Alex J. Walsh, J. Vis. Exp. (177), e63282.
- Osr1 regulates macrophage-mediated liver inflammation in non-alcoholic fatty liver disease progression, Liu L, Liu Z, Zhou Y, Li J, Hu Linghao, He L, Gao G, Kidd B, Alex J Walsh, Jiang R, Wu C, Zhang K, Xie L, Cellular and Molecular Gastroenterology and Hepatology, 2022, 15(5) 1117-1133.