Congratulations to Walsh Lab members Linghao Hu and Daniela de Hoyos, as well as our collaborators Yuanjiu Lei and A. Phillip West (Jackson Laboratory) on our publication in APL Bioengineering, “3D convolutional neural networks predict cellular metabolic pathway use from fluorescence lifetime decay data“. In this paper, we show how label-free autofluroescence lifetime imaging of …
Congratulations to Nianchao Wang, on the successful defense of his Master’s Thesis. His project was to develop a method to compare instance segmentation results, so that different image segmentation algorithms can be quantitatively compared. If you’re interested in learning more about his work, it is published in PlosOne:
The Walsh Lab was busy at SPIE Photonics West this January. Four members of the lab attended, Oscar Benavides, Alex Walsh, Elizabeth Bullard, and Blanche ter Hofstede. Keep an eye out for the online publication of our presentations and proceedings papers! Paper 12827-30PLμTO: a polarized light-sheet microscopy and tomography system for versatile multi-scale and high-throughput …
We have a new publication (https://doi.org/10.3389/fbioe.2023.1293268) on our work related to autofluorescence lifetime microscopy for imaging cellular metabolism. In this paper, we define NAD(P)H and FAD fluorescence lifetime changes with perturbations to inhibit or enhance the metabolic pathways of glycolysis, oxidative phosphorylation, and glutaminolysis. Additionally, we develop and test conventional feature-based machine learning models to …
Congratulations to undergraduate researcher Melody Yeh, who was awarded the Freshman Gathright Scholar award for the College of Engineering. https://tamupkp.wordpress.com/2023/10/02/2023-gathright-deans-excellence-award-winners/ This highly competitive award is sponsored by Chapter 053 of The Honor Society of Phi Kappa Phi and TAMU’s LAUNCH: Academic Excellence. The awards recognize and celebrate outstanding first- and second- year students with exceptional …
Oscar Benavides won 2nd place for his poster at TAMU’s post-doc symposium! Congratulations Oscar!
Multiphoton fluorescence lifetime microscope with tunable TiSapph laser (~680-1080 nm) for excitation. Two channel FLIM imaging plus a third intensity PMT. Inverted configuration with 20X, 0.8 NA air objective, 40X 1.1 NA water objective, and 100X 1.46 NA oil objective. Emission filters include 447/60 nm, 550/88 nm, and 675/67 nm. Environmental control for live cell …
Lightsheet fluorescence microscope with integrated and co-aligned infrared laser. Lightsheet includes 375, 405, 488, 561, and 640 nm excitation lasers. A 10X 0.3 water dipping objective is used for illumination and a 20X water dipping objective is used for imaging. Two cameras allow separate, two color simultaneous imaging. Emission filters include 447/60, 525/50, 513/17, 550/88, …
Customized lightsheet microscopes for fluorescence and scattering tomography.
Custom built diffuse reflectance and fluorescence hyperspectral microscope. Fluorescence excitation at 385 nm.